In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

As a valuable medicinal plant, Salvia plebeia R. Brown (S. plebeia) belongs to the Lamiaceae family that has been subjected over-exploitation in its natural habitat for phytochemical and pharmacological studies. The present study focuses on development of micropropagation protocol sustainable propagation conservation S. plebeia. Direct organogenesis (from shoot tips cotyledonary nodes explants) globular bodies (GBs) induction hypocotyl systems have established Alternative collection methods need be developed large-scale In addition, roots as explants can also induce adventitious shoots via callus. highest number regenerated (7.0 ± 0.8) per was obtained Murashige Skoog (MS) medium supplemented with combination 0.1 mg L−1 indole-3-acetic acid (IAA) 1.0 6-benzyladenine (6-BA), proliferation rooted were carried out same treatments almost synchronously. Similarly, MS IAA thidiazuron (TDZ) yielded maximum (37.5 1.3) 100% sprouting frequency. Simultaneously, GB from explants, it produced 17.4 GBs explant 82.7% response TDZ (1.0 L−1) (0.1 L−1), characterized by globular, heart-shaped, stages successfully germinated hormone-free medium, developmental process which similar embryo. acclimatized plantlets well-developed root shifted soils survival rate. Taken together, is foremost report vitro regeneration plebeia; this work will facilitate germplasm preservation genetic transformation provides necessary plant materials various biotechnological pharmaceutical applications.